citrullinemia (ATCC® CCL-76)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age infant
Gender female
Ethnicity Caucasian
Applications
Deficient in citrulline metabolism.
Karyotype normal diploid female
Clinical Data
From patient with citrullinemia.
female
Caucasian
infant
Comments
From patient with citrullinemia.
Deficient in citrulline metabolism.
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Remove medium, add fresh 0.25% trypsin solution, remove trypsin and let the culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Isoenzymes
G6PD, B
Name of Depositor WJ Mellman
References

. . Proc. Natl. Acad. Sci. USA 57: 829, 1967.

Basic Documentation
References

. . Proc. Natl. Acad. Sci. USA 57: 829, 1967.