E. Derm (NBL-6) (ATCC® CCL-57)

Organism: Equus caballus, horse  / 

Organism Equus caballus, horse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 4 years
Gender female
Strain quarterhorse
Storage Conditions liquid nitrogen vapor temperature
Karyotype model number = 64; range = 52 to 145. The donor's sex (female) is confirmed chromosomally. Although the chromosome numbers vary widely, 55% of the cells have a chromosome number within + or - 1 of the diploid and modal number (64).
Clinical Data
female
Virus Resistance
adenovirus 5; coxsackievirus A9, B5; poliovirus 2
Comments
Cells appear to senesce after 40 passages.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor AJ Kniazeff, WA Nelson-Rees, NB Darby
Passage History
Cells appear to senesce after 40 passages.
Year of Origin September, 1965