RPMI 1846 (ATCC® CCL-49)

Organism: Mesocricetus auratus, hamster, Syrian golden  / 

Permits and Restrictions

View Permits

Organism Mesocricetus auratus, hamster, Syrian golden
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 67; range = 61 to 69
Genes Expressed
melanin
Tumorigenic Yes
Effects
Yes, in newborn hamsters
Virus Susceptibility Vesicular stomatitis virus
Human poliovirus 1
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor GE Moore
References

Moore GE, et al. Continuous culture of a melanotic cell line from the golden hamster. Science 137: 986-987, 1962. PubMed: 14475649

Mount D, et al. Culture of malignant tumors of the Syrian hamster. J. Natl. Cancer Inst. 31: 1217-1237, 1963. PubMed: 14071829

Hsu TC, Kellogg DS Jr.. Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens. J. Natl. Cancer Inst. 25: 221-235, 1960. PubMed: 14403619

Fortner JG, et al. Transplantable tumors of the Syrian (golden) hamster. I. Tumors of the alimentary tract, endocrine glands and melanomas. Cancer Res. 21: 161-198, 1961. PubMed: 13700922

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Moore GE, et al. Continuous culture of a melanotic cell line from the golden hamster. Science 137: 986-987, 1962. PubMed: 14475649

Mount D, et al. Culture of malignant tumors of the Syrian hamster. J. Natl. Cancer Inst. 31: 1217-1237, 1963. PubMed: 14071829

Hsu TC, Kellogg DS Jr.. Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens. J. Natl. Cancer Inst. 25: 221-235, 1960. PubMed: 14403619

Fortner JG, et al. Transplantable tumors of the Syrian (golden) hamster. I. Tumors of the alimentary tract, endocrine glands and melanomas. Cancer Res. 21: 161-198, 1961. PubMed: 13700922