L1210 (ATCC® CCL-219)

Organism: Mus musculus, mouse  /  Tissue: skin  /  Disease: lymphocytic leukemia

Organism Mus musculus, mouse
Tissue skin
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphocytic leukemia
Age 8 months
Gender female
Strain DBA subline 212
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 40; range = 32 to 80.
Mouse karyotype with a diploid stemline number of 40 chromosomes.
Derivation
The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether.
Clinical Data
female

Tumorigenic Yes
Effects
Yes, in nude mice
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 x 10 4 viable cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 4 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Population Doubling Time 8 to 10 hrs
Name of Depositor EM Jensen
References

Law LW, et al. Observations on the effect of a folic-acid antagonist on transplantable lymphoid leukemias in mice. J. Natl. Cancer Inst. 10: 179-192, 1949. PubMed: 18143260

Moore GE, et al. Suspension Cell Culture and In Vivo and In Vitro Chromosome Constitution of Mouse Leukemia L1210. J. Natl. Cancer Inst. 36: 405-421, 1966. PubMed: 18630317

Himmelfarb P, et al. Growth of Colonies of. Murine Leukemia L1210 in Vitro. Cancer Chemother. Rep. 51: 451-3 1967

Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865

Basic Documentation
References

Law LW, et al. Observations on the effect of a folic-acid antagonist on transplantable lymphoid leukemias in mice. J. Natl. Cancer Inst. 10: 179-192, 1949. PubMed: 18143260

Moore GE, et al. Suspension Cell Culture and In Vivo and In Vitro Chromosome Constitution of Mouse Leukemia L1210. J. Natl. Cancer Inst. 36: 405-421, 1966. PubMed: 18630317

Himmelfarb P, et al. Growth of Colonies of. Murine Leukemia L1210 in Vitro. Cancer Chemother. Rep. 51: 451-3 1967

Young SW, et al. Gadolinium(III) texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865