HG-261 (ATCC® CCL-122)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Permits and Restrictions

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 6 years
Gender male
Ethnicity Caucasian
Clinical Data
male
Caucasian
6 years
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution rinse and remove most of the trypsin. Incubate at 37C for 2 to 5 minutes or until the cells loosen. Add fresh medium, aspirate and dispense into new flasks.
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8
vWA: 16,17
Isoenzymes
G6PD, B
Name of Depositor J German
References

. . Ann. Genet. 9: 143, 1966.

Todaro GJ. Variable susceptibility of human cell strains to SV40 transformation. Natl. Cancer Inst. Monogr. 29: 271-275, 1968. PubMed: 4305429

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Ann. Genet. 9: 143, 1966.

Todaro GJ. Variable susceptibility of human cell strains to SV40 transformation. Natl. Cancer Inst. Monogr. 29: 271-275, 1968. PubMed: 4305429