HG-261 (ATCC® CCL-122)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  / 

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 6 years
Gender male
Ethnicity Caucasian
Clinical Data
male
Caucasian
6 years
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution rinse and remove most of the trypsin. Incubate at 37C for 2 to 5 minutes or until the cells loosen. Add fresh medium, aspirate and dispense into new flasks.
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8
vWA: 16,17
Isoenzymes
G6PD, B
Name of Depositor J German
References

. . Ann. Genet. 9: 143, 1966.

Todaro GJ. Variable susceptibility of human cell strains to SV40 transformation. Natl. Cancer Inst. Monogr. 29: 271-275, 1968. PubMed: 4305429

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Ann. Genet. 9: 143, 1966.

Todaro GJ. Variable susceptibility of human cell strains to SV40 transformation. Natl. Cancer Inst. Monogr. 29: 271-275, 1968. PubMed: 4305429