Detroit 573 (ATCC® CCL-117)

Organism: Homo sapiens, human  /  Cell Type: fibroblast, cytogenetic abnormality  / 

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Cell Type fibroblast, cytogenetic abnormality
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age newborn
Gender female
Ethnicity Caucasian
Karyotype B/D translocation; 45,XX,t(Bp-;Dq+); translocation involves chromosomes 5 and 14
Clinical Data
female
Caucasian
newborn
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation