Detroit 548 (ATCC® CCL-116)

Organism: Homo sapiens, human  /  Cell Type: fibroblast, cytogenetic abnormality  / 

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Organism Homo sapiens, human
Cell Type fibroblast, cytogenetic abnormality
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age infant
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype partial D group trisomy; 46,XX,C+,D-; C+ results from D - D translocation
Clinical Data
female
Caucasian
infant
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, sodium pyruvate (1 mM) and lactalbumin hydrolysate (0.1%), 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:11 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isoenzymes
G6PD, B
Name of Depositor CS Stulberg
References

Zuelzer WW, et al. Evidence for a genetic factor related to leukemogenesis and congenital anomalies: chromosomal aberrations in pedigree of an infant with partial D trisomy and leukemia. J. Pediatr. 72: 367-376, 1968. PubMed: 5237794

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Zuelzer WW, et al. Evidence for a genetic factor related to leukemogenesis and congenital anomalies: chromosomal aberrations in pedigree of an infant with partial D trisomy and leukemia. J. Pediatr. 72: 367-376, 1968. PubMed: 5237794