MAT-Ly-Lu-B-2 [MLLB-2] (ATCC® CRL-2376)

Organism: Rattus norvegicus, rat  /  Disease: malignant carcinoma

Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease malignant carcinoma
Age 22 months
Gender male
Strain Copenhagen
Applications
MAT-LyLu was derived from a adult malignant rat prostate tumor obtained from a 22 month old inbred Copenhagen rat by Isaacs et al.
They are more resistant to vinblastine compared to controls.
These cells express a multidrug (MDR) resistant phenotype.
This cell line can be used to study chemotherapy resistance in prostate cancer.
Derivation
MAT-Ly-Lu-B-2 was developed from the MAT-LyLu cell line (ATCC JHU-92). MAT-LyLu was derived from a adult malignant rat prostate tumor obtained from a 22 month old inbred Copenhagen rat by Isaacs et al.
Clinical Data
This cell line can be used to study chemotherapy resistance in prostate cancer.
male
Comments
MAT-Ly-Lu-B-2 was developed from the MAT-LyLu cell line (ATCC JHU-92). MAT-LyLu was derived from a adult malignant rat prostate tumor obtained from a 22 month old inbred Copenhagen rat by Isaacs et al.
The parental cells were grown in increasing concentrations of the drug doxorubicin until the final concentration of 0.001 mM was achieved.
These cells express a multidrug (MDR) resistant phenotype. They are more resistant to vinblastine compared to controls.
This cell line can be used to study chemotherapy resistance in prostate cancer.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.001 mM doxorubicin, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor KJ Pienta
References

Isaacs JT, et al. Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate 9: 261-281, 1986. PubMed: 3774632

Replogle-Schwab TS, et al. Development of doxorubicin resistant rat prostate cancer cell lines. Anticancer Res. 17: 4535-4538, 1997. PubMed: 9494564

Basic Documentation
References

Isaacs JT, et al. Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate 9: 261-281, 1986. PubMed: 3774632

Replogle-Schwab TS, et al. Development of doxorubicin resistant rat prostate cancer cell lines. Anticancer Res. 17: 4535-4538, 1997. PubMed: 9494564