MMQ (ATCC® CRL-10609)

Organism: Rattus norvegicus, rat  / 

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Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties suspension
Biosafety Level 1
Age 5 days
Applications
The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Storage Conditions liquid nitrogen vapor phase
Receptor Expression
dopamine
Genes Expressed
prolactin
Tumorigenic No
Effects
No, in immunosuppressed mice
Yes, in semisolid medium
Comments
The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 2.5%
  • horse serum to a final concentration of 15%
  • Subculturing
    Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10(5) viable cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
    Medium Renewal: Every 2 days
    Cryopreservation
    Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    DGrowth Conditions: The line grows as clumps of cells in suspension.
    Population Doubling Time 24 to 48 hrs
    Name of Depositor University of Virginia Patent Foundation
    References

    MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996

    The MMQ cell line was established from the 7315a rat pituitary tumor.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996

    The MMQ cell line was established from the 7315a rat pituitary tumor.