LTPA (ATCC® CRL-2389)

Organism: Mus musculus, mouse  /  Cell Type: Epithelial  /  Tissue: pancreas  /  Disease: adenocarcinoma

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Organism Mus musculus, mouse
Tissue pancreas
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 12 month old
Gender female
Strain LT/Sv
Storage Conditions liquid nitrogen vapor phase
Karyotype Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Derivation
LTPA is an epithelial cell line derived in 1975 by Edward H. Leiter at The Jackson Laboratory, Bar Harbor, Maine from a spontaneous pancreatic adenocarcinoma taken from a 12 month old female Lt/Sv mouse.
Comments

A defective Polyoma virus infection of a pancreatic duct cell or its precursor appears to represent the neoplastic transforming event.

LTPA cells carry a persistent Polyoma infection.

When injected subcutaneously into Swiss nu/nu mice, LTPA cells formed ductular structures, which were destroyed by inflammatory reactions within 3 weeks.

A culture submitted to the ATCC in May of 1998 was found to be contaminated with mycoplasma and progeny were cured by a 21-day treatment with BM Cycline.

The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell supension to new culture vessels. 
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Complete culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Name of Depositor EH Leiter
Year of Origin 1975
References

Leiter EH, et al. An epithelial cell line with chronic polyoma infection established from a spontaneous mouse pancreatic adenocarcinoma. Cancer Res. 38: 969-977, 1978. PubMed: 205354

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Leiter EH, et al. An epithelial cell line with chronic polyoma infection established from a spontaneous mouse pancreatic adenocarcinoma. Cancer Res. 38: 969-977, 1978. PubMed: 205354

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.