SVR A221a (ATCC® CRL-2386)

Organism: Mus musculus, mouse  /  Cell Type: endothelialSV40 transformed  / 

Organism Mus musculus, mouse
Cell Type endothelialSV40 transformed
Product Format frozen
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Strain C57BL/6
Applications
SVR A221a is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280).
This cell line along with a wild type control SVR bag4 (ATCC CRL-2287) can be used to determine if drugs regulate the MAPKK pathway.
Derivation
SVR A221a is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280).
Tumorigenic Yes
Effects
Yes,
Comments
SVR A221a is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280).
SVR is a hygromycin resistant mouse endothelial cell line containing a temperature sensitive SV40 large T-antigen and H-RAS oncogene.
SVR cells were infected with a retrovirus encoding a dominant negative allele of the Mitogen-Activated Protein Kinase Kinase (MAPKK) gene A221a and selected in puromycin.
RAS oncogenes upregulate two signal transduction pathways, the MAP Kinase Kinase and phosphoinositol-3-Kinase pathways.
Dominant negative MAPKK causes decreased growth in vitro but not in vivo.
This cell line along with a wild type control SVR bag4 (ATCC CRL-2287) can be used to determine if drugs regulate the MAPKK pathway.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 1.0 g/L glucose, 95%; fetal bovine serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JL Arbiser
References

LaMontagne KR Jr., et al. Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566

Basic Documentation
References

LaMontagne KR Jr., et al. Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566