MS1 (MILE SVEN 1) (ATCC® CRL-2279)

Organism: Mus musculus, mouse  /  Cell Type: SV40 transformed  /  Tissue: pancreas/islet of Langerhans; endothelium  / 

Organism Mus musculus, mouse
Tissue
pancreas/islet of Langerhans; endothelium
Cell Type SV40 transformed
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Strain C57BL/6
Applications

This cell line is useful for the study of signal transduction of angiogenic factors.

This cell line is the only line known that can give rise to benign hemangiomas.

 

Storage Conditions liquid nitrogen vapor phase
Derivation
MS1 is a pancreatic islet endothelial cell line established in 1994.
Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.
Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.
Receptor Expression
vascular endothelial growth factor (VEGF), expressed
Cellular Products
tissue inhibitor of bioreactive matrix metalloproteinase (high levels)
Comments
The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.
The expression of high levels of the tissue inhibitor of bioreactive matrix metalloproteinase makes the behavior of this cell line more like that of normal macrophages from some commonly used strains of mice.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor JL Arbiser
Year of Origin 1994
References

Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370

Basic Documentation
References

Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370