TGP55 (ATCC® CRL-2150)

Organism: Mus musculus, transgenic, mouse, transgenic  /  Cell Type: Epithelial,Epithelial-like  /  Disease: pancreatic tumor

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Organism Mus musculus, transgenic, mouse, transgenic
Cell Type Epithelial,Epithelial-like
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 CELLS CONTAIN PAPOVAVIRUS
Disease pancreatic tumor
Age 7.5 months
Gender female
Strain Tg(E1a-1-SV40E)Bri18
Applications
TGP55 is an epithelial-like cell line derived from a pancreatic tumor with small cell anaplastic morphology that arose in an adult female transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the coding region for the SV40 T antigen.
Derivation
TGP55 is an epithelial-like cell line derived from a pancreatic tumor with small cell anaplastic morphology that arose in an adult female transgenic mouse.
Clinical Data
TGP55 is an epithelial-like cell line derived from a pancreatic tumor with small cell anaplastic morphology that arose in an adult female transgenic mouse.
female
Genes Expressed
insulin; somatostatin
Comments
TGP55 is an epithelial-like cell line derived from a pancreatic tumor with small cell anaplastic morphology that arose in an adult female transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the coding region for the SV40 T antigen.
The cells do not become confluent.
They grow very slowly as islands, and should be subcultured when the islands begin to form domes.
Complete Growth Medium A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor OS Pettengill, D Longnecker
References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904