xrs5 (ATCC® CRL-2348)

Organism: Cricetulus griseus, hamster, Chinese  / 

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender female
Applications
xrs5 is a X-ray sensitive Chinese Hamster Ovary mutant cell line which was derived from CHO-K1 cells by treating the cells with ethyl methanesulphonate and subsequent growth in agar.
Derivation
xrs5 is a X-ray sensitive Chinese Hamster Ovary mutant cell line which was derived from CHO-K1 cells by treating the cells with ethyl methanesulphonate and subsequent growth in agar.
Clinical Data
female
Comments
xrs5 is a X-ray sensitive Chinese Hamster Ovary mutant cell line which was derived from CHO-K1 cells by treating the cells with ethyl methanesulphonate and subsequent growth in agar.
These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen.
Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination.
The cells will revert to wild type on treatment with azacytidine.
Complete Growth Medium Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor G Chu
References

Jeggo PA, Kemp LM. X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents. Mutat. Res. 112: 313-327, 1983. PubMed: 6197643

Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631

Rathmell WK, Chu G. A DNA end-binding factor involved in double-strand break repair and V(D)J recombination. Mol. Cell. Biol. 14: 4741-4748, 1994. PubMed: 7516471

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Jeggo PA, Kemp LM. X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents. Mutat. Res. 112: 313-327, 1983. PubMed: 6197643

Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631

Rathmell WK, Chu G. A DNA end-binding factor involved in double-strand break repair and V(D)J recombination. Mol. Cell. Biol. 14: 4741-4748, 1994. PubMed: 7516471