OV-90 (ATCC® CRL-11732)

Organism: Homo sapiens, human  /  Tissue: ovary; derived from metastatic site: ascites  /  Disease: grade 3, stage IIIC, malignant papillary serous adenocarcinoma

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Organism Homo sapiens, human
Tissue
ovary; derived from metastatic site: ascites
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease grade 3, stage IIIC, malignant papillary serous adenocarcinoma
Age 64 years
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype 46, XX, der(1)t(1;10)(p36;p15), hsr(3)(p11), der(9;17)(q10;q10), der(10)t(10;17)(p15;p12p13), der(13)t(13;13)(p11;q14) RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Derivation
This cell line was initiated in August of 1992 from a patient of French-Canadian descent with no family history of ovarian cancer.
Clinical Data
female
Oncogene her2/neu +, p53 (mutated, Ser --> Arg mutation at exon 6, codon 215) RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Genes Expressed
keratin RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Tumorigenic Yes
Effects
Yes, the cells are tumorigenic in nude mice and form colonies in soft agar
Comments
Like TOV-21G (ATCC CRL-11730), the OV-90 (ATCC CRL-11732) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731) cell line does not exhibit a deletion at chromosome 3p24.
Complete Growth Medium The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Subculturing
    Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
      Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
      Medium Renewal: Every 3 to 4 days
      Cryopreservation
      Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage Temperature: Liquid nitrogen vapor phase
      Culture Conditions
      Temperature: 37°C
      Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
      STR Profile
      Amelogenin: X
      CSF1PO: 12,13
      D13S317: 11,12
      D16S539: 11
      D5S818: 11,15
      D7S820: 10,10.1
      THO1: 9.3
      TPOX: 8,10
      vWA: 16,17
      Name of Depositor University of Montreal
      Year of Origin August, 1992
      References

      Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

      Lounis H, et al. Mapping of chromosome 3p deletions in human epithelial ovarian tumors. Oncogene 17: 2359-2365, 1998. PubMed: 9811467

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

      Notice: Necessary PermitsPermits

      These permits may be required for shipping this product:

      • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
      Basic Documentation
      References

      Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

      Lounis H, et al. Mapping of chromosome 3p deletions in human epithelial ovarian tumors. Oncogene 17: 2359-2365, 1998. PubMed: 9811467

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

      Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993