LA 3-5 (ATCC® CRL-10101)

Organism: Cricetulus griseus, hamster, Chinese  / 

Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender female
Applications
The cells are resistant to methotrexate.
Clinical Data
female
Comments
The cells are resistant to methotrexate.
Complete Growth Medium Alpha minimum essential medium with 0.001 mM methotrexate, 90%; dialyzed heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Rinse cell sheet 2 times with fresh 0.25% trypsin, remove trypsin and allow the culture to stand for 5 to 10 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor Genetics Institute, Inc.