MC-SV-HUC T-2 (ATCC® CRL-9519)

Organism: Homo sapiens, human  / 

Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age 11 years
Gender male
Applications
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.
Clinical Data
male
Genes Expressed
uroepithelial keratins
Cellular Products
uroepithelial keratins
Tumorigenic Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Comments
The cells were initially transformed with SV40 virus, and later treated with 3-methylcholanthrene (MCA).
While the parental line (SV-HUC-1, see ATCC CRL-9520) was not tumorigenic and had a balanced chromosome composition, the MCA treated line became tumorigenic and aneuploid.
The line has been repeatedly tested for production of infectious SV40 using an African Green Monkey kidney cell plaque assay, and has always tested negative.
Stress (such as chemical exposure) could possibly activate the virus.
Complete Growth Medium Ham's F12 medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Wisconsin Alumni Res. Fndn.
References

Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990

Basic Documentation
References

Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990