SCC-4 (ATCC® CRL-1624)

Organism: Homo sapiens, human  /  Tissue: tongue  /  Disease: squamous cell carcinoma

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Organism Homo sapiens, human
Tissue tongue
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1
Disease squamous cell carcinoma
Age 55 years
Gender male
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Genes Expressed
epidermal keratins; 40 kD keratin
Tumorigenic Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments
SCC-4 forms colonies in semi-solid medium, and is not induced to differentiate by anchorage deprivation.
Clonal growth of these cells is improved by using a 3T3  (ATCC CCL-92) feeder layer (see Rheinwald and Green, Cell 6:331, 1975 for methods) RefRheinwald JG, Green H. Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma. Cell 6: 317-330, 1975. PubMed: 1052770.

ATCC grows these cells on 56-X.2, MITC-STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 106/T75 in order to obtain a 30% confluent monolayer.

Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
  • 400 ng/ml hydrocortisone
  • fetal bovine serum to a final concentration of 10%.

Subculturing

Note: Subculture before 100% confluent.

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). 
  4. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  5. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  6. Add appropriate aliquots of the cell suspension to new culture vessels pre-plated with ATCC 56-X.2 feeder layer (MITC-STO cells).
  7. Inoculate new flasks with 3 x 103 cells/cm2.
  8. Incubate cultures at 37°C.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.  

Medium Renewal
Two to three times weekly

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,13
D16S539: 12
D5S818: 13
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 15,17
Name of Depositor JG Rheinwald
References

Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336

Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336

Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916