MTC-M (ATCC® CRL-1806)

Organism: Mus musculus, mouse  /  Cell Type: C cell  /  Tissue: thyroid  / 

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Organism Mus musculus, mouse
Tissue thyroid
Cell Type C cell
Product Format frozen
Morphology epithelial
Culture Properties suspension
Biosafety Level 1
Age 20 months
Gender female
Strain BALB/c
Derivation
This line was derived from a transplantable neoplasm of thyroid C cells.
Clinical Data
female
Genes Expressed
somatostatin; calcitonin
Cellular Products
somatostatin; calcitonin
Comments

The cells can be propagated in spinner cultures or in static cultures.

The amounts of stored and released somatostatin can be increased 2 to 3 fold by treatment for 4 days with 1 micromolar 5-bromo-2'-deoxyuridine.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 85%; heat-inactivated horse serum, 10%; fetal bovine serum, 5%
Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 3 X 106 cells/mL. Cell clumps should be dispersed using a small bore pipette.

Medium Renewal: Every 2 to 3 days

Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Population Doubling Time 96 hrs
Name of Depositor AS Tischler
References

Tischler AS, et al. Establishment of a continuous somatostatin-producing line of medullary thyroid carcinoma cells from BALB/c mice. J. Endocrinol. 110: 309-313, 1986. PubMed: 2875121

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Tischler AS, et al. Establishment of a continuous somatostatin-producing line of medullary thyroid carcinoma cells from BALB/c mice. J. Endocrinol. 110: 309-313, 1986. PubMed: 2875121