CGBQ (ATCC® CCL-169)

Organism: Anser anser, goose  /  Cell Type: fibroblast  / 

Organism Anser anser, goose
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 15 to 17 days gestation
Storage Conditions liquid nitrogen vapor phase
Karyotype range 80 to 145
Exact chromosome count to determine the modal number is difficult to achieve owing to the large number of microchromosomes which complicate the picture. There are 20-27 macrochromosomes.
Virus Susceptibility Herpes simplex virus
Sindbis virus
Vesicular stomatitis virus
Human poliovirus 1
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) glycerol
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor HG Coon
References

. . Carnegie Inst. Wash. Year Book 67: 421, 1968.

Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097

Basic Documentation
References

. . Carnegie Inst. Wash. Year Book 67: 421, 1968.

Coon HG, Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097