S16Y (ATCC® CRL-2943)

Organism: Rattus norvegicus, rat  /  Cell Type: Schwann cell  /  Tissue: sciatic nerve  / 

Organism Rattus norvegicus, rat
Tissue sciatic nerve
Cell Type Schwann cell
Product Format frozen
Morphology spindle-shaped
Culture Properties adherent
Biosafety Level 1
Age neonatal
Applications
16Y, S16 (ATCC CRL-2941) and S42 (ATCC CRL-2942) cells should be useful for investigating the cell biology of MAG and other myelin-related components as the levels of MAG expression in the three lines are inversely related to their rates of proliferation.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The S16Y cell line arose spontaneousely from a passage of the S16 (ATCC CRL-2941), a line that was derived from a primary culture of Schwann cells and was immortalized by repetitive passaging.
Antigen Expression
Myelin-associated glycoprotein (MAG), not expressed
Galactocerebroside (GalC), not expressed
P0 glycoprotein, not expressed
Genes Expressed
Myelin-associated glycoprotein (MAG), not expressed,Galactocerebroside (GalC), not expressed,P0 glycoprotein, not expressed
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Note: The culture flasks should be treated with 0.1mL/cmof flask surface area with 15 µg/mL poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells. 
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (DPBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g  for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37°C.

Subcultivation ratio: A subcultivation ratio of 1:5 to 1:12 twice weekly is recommended.
Medium renewal: Every 2 to 3 days.
Cryopreservation
Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time approximately 20 hours
Name of Depositor RH Quarles
Year of Origin 1989
References

Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597

Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958

Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295

Basic Documentation
Other Documentation
References

Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597

Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958

Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295