S16 (ATCC® CRL-2941)

Organism: Rattus norvegicus, rat  /  Cell Type: Schwann cell  /  Tissue: sciatic nerve  / 

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Organism Rattus norvegicus, rat
Tissue sciatic nerve
Cell Type Schwann cell
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1
Age neonatal
Applications
In many respects, the S16 and S42 (ATCC CRL-2942) cells, biochemically, resemble Schwann cells at an early stage in their preparation to myelinate and should be useful for investigating the cell biology of MAG and other myelin-related components.

Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The S16 cell line is derived from a primary culture of Schwann cells that was immortalized by repetitive passaging. ­
Antigen Expression
Myelin-associated glycoprotein (MAG)
Galactocerebroside (GalC)
Genes Expressed
laminin,Myelin-associated glycoprotein (MAG),Galactocerebroside (GalC)
Cellular Products
laminin
Comments
High Myelin-associated glycoprotein (MAG) expression was demonstrated by Radioimmunoassay. The S16 cells divide very rapidly, are rounder than normal Schwann cells and elaborate many processes after reaching high density. The cells express galactocerebroside (GalC), sulfatide and P0 glycoprotein, but little or no myelin basic protein.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Note:

The culture flasks should be treated with 0.1 mL/cm2 of flask surface area with 15ug/mL poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (DPBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 4 X 103 to 6 X 104 viable cells/cm2 is recommended.
  7. Incubate cultures at 37°C. We recommend that you maintain cultures at a cell concentration between 2 X 104 and 4 X 104 cells/cm2.

Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 twice weekly is recommended.
Medium renewal: Every 2 to 3 days
Interval: Subculture when cell concentration is between 2 x104 and 4x104 cells/cm2.
Cryopreservation
Freeze medium: complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time approximately 40 hours
Name of Depositor RH Quarles
Year of Origin 1989
References

Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958

Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597

Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958

Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597

Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295