sNF94.3 (ATCC® CRL-2886)

Organism: Homo sapiens, human  /  Cell Type: Schwann Cell  /  Tissue: derived from metastatic site: lung  /  Disease: neurofibrmatosis type 1 (NF1)

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Organism Homo sapiens, human
Tissue derived from metastatic site: lung
Cell Type Schwann Cell
Product Format frozen
Morphology Schwann cell-like
Culture Properties adherent
Biosafety Level 1
Disease neurofibrmatosis type 1 (NF1)
Age 43 year old
Gender female
Storage Conditions liquid nitrogen vapor phase
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Derivation
The sNF94.3 cell line was derived from a lung metastasis diagnosed by histopathology as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both the cytoplasmic Schwann cell markers S100 and p75.
Clinical Data
female
43 years
Comments
This cell line has a normal karyotype. Western immunoblotting showed apparently full-length neurofibromin protein.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 X 103 to 7 X 103 viable cells/cm2 is recommended.
  7. Incubate cultures at 37°C. Subculture when cell concentration is between 8 X 104 and 1 X 105 cells/cm2

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 twice weekly is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Culture medium,90%; DMSO,10%
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 9,12
D5S818: 10,12
D7S820: 9,14
THO1: 7,9.3
TPOX: 11,12
vWA: 17,18
Population Doubling Time about 43 hours
Name of Depositor DF Muir, MR Wallace
Passage History
The cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both the cytoplasmic Schwann cell markers S100 and p75.
Year of Origin 1994
References

Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937

Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Fieber LA, et al. Delayed rectifier K currents in NF1 Schwann cells. Pharmacological block inhibits proliferation. Neurobiol. Dis. 13: 136-146, 2003. PubMed: 12828937

Li Y, et al. Notch and Schwann cell transformation. Oncogene 23: 1146-1152, 2004. PubMed: 14762442