CHLA-01R-MED (ATCC® CRL-3034)

Organism: Homo sapiens, human  /  Tissue: Brain,  Derived from Metastatic site: pleural fluid  /  Disease: medulloblastoma

Organism Homo sapiens, human
Tissue Brain,  Derived from Metastatic site: pleural fluid
Morphology rounded
Culture Properties single cells and tight clusters in suspension
Biosafety Level 1
Disease medulloblastoma
Age 8 years
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human cell line of male origin with a karyotype of: 46,XY,del(11)(q13.3). The chromosome deletion was present in all of the examined cells.
Images CRL-3034 Micrograph
Derivation CHLA-01R-MED was established from an 8 year old boy with recurrent metastatic medulloblastoma   Cells were isolated from pleural fluid at time of recurrence and cultured in neurobasal medium after concentration.

A primary medulloblastoma cell line from this patient is available as CHLA-01-MED (see ATCC CRL-3021)

Genes Expressed INI-1, expressed
Comments

ATCC has verified that CHLA-01R-MED expresses INI-1 and does not express epithelial membrane antigen (EMA1).

This cell line has been continuously carried in culture for over 9 months, and in neurobasal medium grows as spheres of varying sizes.

A primary medulloblastoma cell line from this patient is available as CHLA-01-MED (see ATCC CRL-3021)

The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community. 


 

Complete Growth Medium DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
Subculturing Note: Due to large, tight clusters formed by these cells, it may be difficult to accurately measure cell number and viability.
Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended.
Medium renewal: Add fresh medium twice weekly (depending on cell density).
Cryopreservation Freeze medium: FBS, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11, 13
D13S317: 8, 14
D7S820: 10
D16S539: 9, 11
vWA: 16
TH01: 9, 12
Amelogenin: X Y
TPOX: 9, 12
CSF1PO: 11
Name of Depositor A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles