4/4 R.M.-4 (ATCC® CCL-216)

Organism: Rattus norvegicus, rat  / 

Permits and Restrictions

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Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 2 to 3 months
Gender female
Clinical Data
female
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Remove medium, rinse cell sheet once with fresh 0.25% trypsin and let the culture sit for 10 to 12 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JF Aronson
References

Aronson JF, Cristofalo VJ. Growth of epithelial cells from rat pleura. In Vitro 15: 214, 1979.

Aronson JF, Cristofalo VJ. Culture of epithelial cells from the rat pleura. In Vitro 17: 61-70, 1981. PubMed: 7216240

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Aronson JF, Cristofalo VJ. Growth of epithelial cells from rat pleura. In Vitro 15: 214, 1979.

Aronson JF, Cristofalo VJ. Culture of epithelial cells from the rat pleura. In Vitro 17: 61-70, 1981. PubMed: 7216240