JEG-3 (ATCC® HTB-36)

Organism: Homo sapiens, human  /  Tissue: placenta  /  Disease: choriocarcinoma

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Organism Homo sapiens, human
Tissue placenta
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease choriocarcinoma
Applications

This cell line is a suitable transfection host.

Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypertriploid human cell line. The modal chromosome number is 71, occurring at 34%, and polyploidy at 2.6%. The t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and 6 other markers are common to most cells, and two other markers are found in some. Giant satellites are seen in one N14, and two N22. N2, N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single copy. A single Y chromosome is detected by Q-band examination.
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Derivation
This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates.
Genes Expressed
human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone
Tumorigenic Yes
Effects
Yes, in nude mice; forms malignant tumor consistent with choriocarcinoma
Comments
The cells are able to transform steroid precursors to estrone and estradiol.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 1
PGM3, 1-2
Name of Depositor G Kohler
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

. . Acta Endocrinol. Suppl. 153: 137-153, 1971.

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491

Kohler PO, Bridson WE. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32: 683-687, 1971.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

. . Acta Endocrinol. Suppl. 153: 137-153, 1971.

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Roesler WJ, et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491

Kohler PO, Bridson WE. Isolation of hormone-producing clonal lines of human choriocarcinoma. J. Clin. Endocrinol. 32: 683-687, 1971.