JAR (ATCC® HTB-144™) Organism: Homo sapiens, human / Tissue: placenta / Disease: choriocarcinoma General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Organism Homo sapiens, human Tissue placenta Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Disease choriocarcinoma Age fetus Gender male Ethnicity Caucasian Storage Conditions liquid nitrogen vapor phase Karyotype This is probably a pseudotriploid human cell line with the modal chromosome number of 68, occurring in 24% of cells, but cells with both 69 (22%) and 70 (18%) chromosome counts also occurred frequently. Cells with higher ploidies occurred at 7.0%.Karyotypes were extremely complex. Consistently there were 20 to 25 marker chromosomes (>30% of total chromosome content) per cell. Most marker chromosomes had complex structural rearrangements, and the origin of chromosome segments of these markers often defied identification. Among the frequently found markers were 8p+, 11p, a large metacentric [?t(3qter--3q12::?--C--?::3q12--3qter)] der(?13)T(1;?13) (p13;?q14), and many others. There was only one normal X chromosome. Normal N3 and N13 were not found. Derivation The JAR line was established by R. A. Pattillo and associates directly from a trophoblastic tumor of the placenta. Clinical Data Caucasianfetusmale Genes Expressed estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/mL after reculturing Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Twice per week Cryopreservation Freeze medium: Culture medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C STR Profile Amelogenin: X,YCSF1PO: 7,10D13S317: 11D16S539: 9,10D5S818: 10,11D7S820: 10,11THO1: 6,7TPOX: 8,11vWA: 16,18 Isoenzymes AK-1, 1ES-D, 2G6PD, BGLO-I, 1PGM1, 1-2PGM3, 1-2 Name of Depositor RA Pattillo References Pattillo RA, et al. The Jar cell line -- continuous human multihormone production and controls. In Vitro 6: 398-399, 1971. Basic Documentation Product Sheet Certificate of Analysis SDS References Pattillo RA, et al. The Jar cell line -- continuous human multihormone production and controls. In Vitro 6: 398-399, 1971.