NU-DUL-1 (ATCC® CRL-2969)

Organism: Homo sapiens, human  /  Cell Type: lymphocyte  /  Tissue: peritonial effusion; derived from metastatic site: malignant cerebrospinal fluid

 /  Disease: undifferentiated lymphoma, non-Burkitt's type

Organism Homo sapiens, human
Tissue peritonial effusion; derived from metastatic site: malignant cerebrospinal fluid

Cell Type lymphocyte
Product Format frozen
Morphology early B-cell
Culture Properties suspension
Biosafety Level 1
Disease undifferentiated lymphoma, non-Burkitt's type
Age 43 years
Gender male
Ethnicity Caucasian
Applications The Nu-DUL-1 cell line is a valuable resource for studying the biological behavior of undifferentiated lymphoma cells.
The Nu-DUL-1 cell line will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements.
Storage Conditions liquid nitrogen vapor phase
Karyotype 44, X (hypodiploid), +der(4)t(4;?)(q31;?). -6, -17, -18, -21, +2mar,der(1)t(1;?)(q32;?), del(4)(q25q31), del(5)(p12), del(6)(q21), del(8)(q24), der(11)t(11;?)(q21;?), der(12)t(12;?)(p13;?), der(14)t(14;?)(q13;), ter rea(19;21)(q13;q22)
Images CRL-2969 Micrograph
Clinical Data 43 years
Caucasian
Male
Antigen Expression LN-1+
LN-2+
LN-3+(anti-HLA-Dr)
BA-1+ (CD24)
CALLA (J5)(common ALL antigen; CD10)+
BA-2 - (CD9)
OKM1 - (myeloid marker)
Leu-7 - (natural killer T cell marker)
OKT-3 -, OKT-4 -, OKT-6 -, OKT-8 -, OKT-11 - (T cell markers)
BM-1 -, BM-2 -, BM-3 - ,BM-4 -, BM-5 - (myeloid markers)

EBNA - (Epstein-Barr Nuclear Antigen)
Genes Expressed sIg -
Comments The depositor states that this cell line is negative for the Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.

The NU-DUL-1 cell line represents a very unusual undifferentiated lymphoma.
The NU-DUL-1 cell line has a chromosome 14 translocation, but not from chromosome 11 or 18 and may represent a new variant.
This cell line is hypodiploid with the presumed loss of a Y chromosome.
NU-DUL-1 cells express markers of early B-cell differentiation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 2.0 x 105to 3.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended.
Medium renewal: every 2 to 3 days
Cryopreservation Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 12, 14
D13S317: 8, 13
D7S820: 11, 13
D16S539: 11, 13
vWA: 16, 17
THO1: 9, 9.3
Amelogenin: X
TPOX: 8
CSF1PO: 11, 12

Name of Depositor A Epstein
Year of Origin 1985
References

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Basic Documentation
Other Documentation
References

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794