B14FAF28-G3 (ATCC® CCL-14)

Organism: Cricetulus griseus, hamster, Chinese  / 

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender female
Clinical Data
female
Virus Resistance
poliovirus 1, 3; adenovirus 3
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor TC Hsu
References

. . Science 133: 1600, 1961.

Hsu TC, et al. Radiosensitivity of cells of Chinese hamster in vitro in relation to the cell cycle. Exp. Cell Res. 27: 441-452, 1962. PubMed: 13955320

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Science 133: 1600, 1961.

Hsu TC, et al. Radiosensitivity of cells of Chinese hamster in vitro in relation to the cell cycle. Exp. Cell Res. 27: 441-452, 1962. PubMed: 13955320