B14FAF28-G3 (ATCC® CCL-14)

Organism: Cricetulus griseus, hamster, Chinese  / 

Permits and Restrictions

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Gender female
Clinical Data
female
Virus Resistance
poliovirus 1, 3; adenovirus 3
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor TC Hsu
References

. . Science 133: 1600, 1961.

Hsu TC, et al. Radiosensitivity of cells of Chinese hamster in vitro in relation to the cell cycle. Exp. Cell Res. 27: 441-452, 1962. PubMed: 13955320

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Science 133: 1600, 1961.

Hsu TC, et al. Radiosensitivity of cells of Chinese hamster in vitro in relation to the cell cycle. Exp. Cell Res. 27: 441-452, 1962. PubMed: 13955320