HEPM (ATCC® CRL-1486)

Organism: Homo sapiens, human  /  Tissue: palatal mesenchyme  / 

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue palatal mesenchyme
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age fetus
Gender female
Applications This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a human diploid cell line with the 46,XX karyotype. The modal chromosome number was 46, occurring in 76% of cells. The rate of cells with polyploidies was 4.2%. No consistent chromosome aberrations were detected.
Derivation
HEPM was derived from mesenchymal cells from the human embryo by M Macy.
Clinical Data
female
fetus
Receptor Expression
epidermal growth factor (EGF)
Comments
The cells are highly responsive to epidermal growth factor (EGF).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: 2 to 3 times per week
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 10,11
    D13S317: 8,12
    D16S539: 11,12
    D5S818: 11,13
    D7S820: 8,10
    THO1: 6,9.3
    TPOX: 8,11
    vWA: 17,18
    Name of Depositor M Macy
    Year of Origin July, 1979
    References

    Yoneda T, Pratt RM. Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor. Science 213: 563-565, 1981. PubMed: 7017936

    Yoneda T, Pratt RM. Interaction between glucocorticoids and epidermal growth factor in vitro in the growth of palatal mesenchymal cells from the human embryo. Differentiation 19: 194-198, 1981. PubMed: 6458523

    Yoneda T, Pratt RM. Glucocorticoid receptors in palatal mesenchymal cells from the human embryo: relevance to human cleft palate formation. J. Craniofacial Genet. Dev. Biol. 1: 411-423, 1981.

    Pratt RM, et al. Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate. Teratog. Carcinog. Mutagen. 2: 313-318, 1982. PubMed: 6130630

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Yoneda T, Pratt RM. Mesenchymal cells from the human embryonic palate are highly responsive to epidermal growth factor. Science 213: 563-565, 1981. PubMed: 7017936

    Yoneda T, Pratt RM. Interaction between glucocorticoids and epidermal growth factor in vitro in the growth of palatal mesenchymal cells from the human embryo. Differentiation 19: 194-198, 1981. PubMed: 6458523

    Yoneda T, Pratt RM. Glucocorticoid receptors in palatal mesenchymal cells from the human embryo: relevance to human cleft palate formation. J. Craniofacial Genet. Dev. Biol. 1: 411-423, 1981.

    Pratt RM, et al. Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate. Teratog. Carcinog. Mutagen. 2: 313-318, 1982. PubMed: 6130630