CHO-K1 (ATCC® CRL-9618)

Organism: Cricetulus griseus, hamster, Chinese  /  Tissue: ovary  / 

Permits and Restrictions

View Permits

Organism Cricetulus griseus, hamster, Chinese
Tissue
ovary
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender female
Applications
This cell line is a suitable transfection host.
Clinical Data
female
Comments
This is the same line as ATCC CCL-61.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Once or twice between subculture
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor K Thomae
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation