MLTC-1 (ATCC® CRL-2065)

Organism: Mus musculus, mouse  /  Cell Type: Leydig cell  /  Tissue: testis  /  Disease: Leydig cell tumor

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Tissue testis
Cell Type Leydig cell
Product Format frozen
Morphology epithelial
Culture Properties adherent, Cells form islands that dome and the monolayer may not become confluent.
Biosafety Level 1
Disease Leydig cell tumor
Gender male
Strain C57BL/6
Applications This cell line is a suitable transfection host.
Karyotype polyploid; modal number = 95 RefRebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740
Derivation
This line was established from the M548OP transplantable Leydig cell tumor carried in C57BL/6 mice.RefRebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740
Clinical Data
male
Receptor Expression
chorionic gonadotropin; luteinizing hormone (LH)
Genes Expressed
progesterone
Tumorigenic Yes
Effects
Yes, in C57BL/6 mice
Comments
The cells retain the hormonal responsiveness of the original tumor.
Membrane adenyl cyclase activity is stimulated by treatment with human chorionic gonadotropin (hCG), luteinizing hormone, cholera toxin, sodium fluoride and guanyl-5'-ylimidodiphosphate, and produce progesterone in response to hCG. RefRebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Population Doubling Time 35 to 40 hrs
Name of Depositor RV Rebois
References

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740

Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740