GC-1 spg (ATCC® CRL-2053)

Organism: Mus musculus, transgenic for SV40 early region, mouse, transgenic for SV40 early region  /  Cell Type: transformed with pSV3-neo  /  Tissue: testis  / 

Organism Mus musculus, transgenic for SV40 early region, mouse, transgenic for SV40 early region
Tissue testis
Cell Type transformed with pSV3-neo
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain SV-40 viral DNA sequences]
Age 10 days
Gender male
Strain BALB/c
Storage Conditions liquid nitrogen vapor phase
Derivation Type B spermatogonia were immortalized by transfection with pSV3-neo (a plasmid containing coding sequences for the SV40 large T antigen and neomycin resistance).
Clinical Data
male
Comments
The line shows characteristics of a stage between type B spermatogonia and primary spermatocytes.

The cells express two testis specific isoproteins, cytochrome c and lactate dehydrogenase C4.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
    Medium Renewal: 1 to 2 times per week
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor MC Hofmann
    References

    Hofmann MC, et al. Immortalization of germ cells and somatic testicular cells using the SV40 large T antigen. Exp. Cell Res. 201: 417-435, 1992. PubMed: 1322317

    Basic Documentation
    References

    Hofmann MC, et al. Immortalization of germ cells and somatic testicular cells using the SV40 large T antigen. Exp. Cell Res. 201: 417-435, 1992. PubMed: 1322317