TM3 (ATCC® CRL-1714)

Organism: Mus musculus, mouse  /  Cell Type: Leydig cell  /  Tissue: testis  / 

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Organism Mus musculus, mouse
Tissue testis
Cell Type Leydig cell
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 11 to 13 days
Gender male
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Receptor Expression
luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Genes Expressed
prostaglandin F2a
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
The TM3 cell line responds to LH with an increase in cAMP production, but does not respond to follicle stimulating hormone (FSH).
The maintenance of responsiveness to LH is dependent upon serum lot.
In the presence of LH, the cells are capable of metabolizing cholesterol.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5mM L-Glutamine, 0.5mM Sodium Pyruvate, 1.2 g/L Sodium Bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing
Remove medium, add fresh 0.25% trypsin-0.53mM EDTA solution, rinse, remove trypsin and let the culture sit at room temperature for 2 to 4 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:200 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium, 95%, DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Name of Depositor JP Mather
References

Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781

Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781

Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561