TOV-112D (ATCC® CRL-11731)

Organism: Homo sapiens, human  /  Tissue: Ovary  /  Disease: grade 3, stage IIIC, primary malignant adenocarcinoma; endometrioid carcinoma

Organism Homo sapiens, human
Tissue
Ovary
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease grade 3, stage IIIC, primary malignant adenocarcinoma; endometrioid carcinoma
Age 42 years adult
Gender female
Storage Conditions liquid nitrogen vapor temperature
Karyotype 52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Images
Derivation
This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer.
Clinical Data
female
This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer.
Oncogene her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175) RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
Genes Expressed

keratin

Cellular Products
keratin
Tumorigenic Yes
Effects
The cells are tumorigenic in nude mice and form colonies in soft agar
Comments
Unlike TOV-21G (ATCC CRL-11730) and OV-90 (ATCC CRL-11732), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24.
Complete Growth Medium The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Subculturing
    Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
    Medium Renewal: Every 3 to 4 days
    Cryopreservation
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor temperature
    Culture Conditions
    Temperature: 37°C
    STR Profile
    Amelogenin: X
    CSF1PO: 12
    D13S317: 8
    D16S539: 9,12
    D5S818: 10
    D7S820: 9,10
    THO1: 6
    TPOX: 8,11
    vWA: 18
    Name of Depositor University of Montreal
    Year of Origin 1992
    References

    Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Basic Documentation
    Other Documentation
    References

    Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

    Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993