6E6 (ATCC® CRL-11398)

Organism: Cricetulus griseus, hamster, Chinese  / 

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Organism Cricetulus griseus, hamster, Chinese
Product Format frozen
Morphology epithelial
Culture Properties loosely adherent
Biosafety Level 2 Cells contain SV-40-CMV viral DNA sequences
Gender female
Applications
The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
The cell line is capable of producing about 5-10 micrograms/liter of the humanized immunoglobulin at the rate of 1 ng/cell/day.
Storage Conditions liquid nitrogen vapor phase
Derivation
The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
Clinical Data
female
Comments
This line is Neomycin resistant.
The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).
The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences.
The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte integrins.
The cell line is capable of producing about 5-10 micrograms/liter of the humanized immunoglobulin at the rate of 1 ng/cell/day.
Complete Growth Medium A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5 mM L-glutamine, 95%; heat-inactivated fetal bovine serum, 5%
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype IgG4 kappa
Name of Depositor ICOS Corporation
References

Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998

Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158

Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998

Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158

Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282