R2C (ATCC® CCL-97)

Organism: Rattus norvegicus, rat  / 

Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 2 months
Gender male
Strain WFu
Applications
transfection host
Clinical Data
male
Genes Expressed
steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Cellular Products
steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Virus Resistance
poliovirus 2
Comments
Stimulation by other hormones or cAMP is not required for hormone secretion.
Complete Growth Medium The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 2.5%
  • horse serum to a final concentration of 15%

Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Add fresh medium at least twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor G Sato
References

Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022

Basic Documentation
References

Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022