LC-540 (ATCC® CCL-43)

Organism: Rattus norvegicus, rat  / 

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Organism Rattus norvegicus, rat
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender male
Strain Fischer
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 42; range = 40 to 43.
Approximately 21% of the cells examined were aneuploids. No other structural abnormalities were noted.
Clinical Data
male
Tumorigenic Yes
Effects
Yes, in rats
Virus Susceptibility Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
Virus Resistance
poliovirus 1
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor A Steinberger
Year of Origin October, 1969
References

Jacobs BB, Huseby RA. Transplantable Leydig cell tumors in Fischer rats: hormone responsibility and hormone production. J. Natl. Cancer Inst. 41: 1141-1153, 1968. PubMed: 5688092

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Jacobs BB, Huseby RA. Transplantable Leydig cell tumors in Fischer rats: hormone responsibility and hormone production. J. Natl. Cancer Inst. 41: 1141-1153, 1968. PubMed: 5688092