HGF-1 (ATCC® CRL-2014)

Organism: Homo sapiens, human  /  Tissue: gingival biopsy  /  Disease: normal

Organism Homo sapiens, human
Tissue gingival biopsy
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease normal
Age 28 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
­
Clinical Data
28 years
Caucasian
male
Receptor Expression
bradykinin, expressed
Comments

The cells were deposited to ATCC at an unknown passage level. A longevity study performed by ATCC showed that the depositor's material was capable of growing for an additional 22 population doublings (i.e., unknown + 22) before the onset of senescence.

Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells.

Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,13
D5S818: 12
D7S820: 10
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Name of Depositor BS McAllister
Passage History
The cells were deposited to ATCC at an unknown passage level
Year of Origin October, 1989
References

McAllister BS, et al. Bradykinin receptors and signal transduction pathways in human fibroblasts: integral role for extracellular calcium. Arch. Biochem. Biophys. 304: 294-301, 1993. PubMed: 7686736

McAllister BS, et al. Bradykinin inhibition of EGF- and PDGF-induced DNA synthesis in human fibroblasts. Am. J. Physiol. 265: C477-C484, 1993. PubMed: 8396328

Basic Documentation
References

McAllister BS, et al. Bradykinin receptors and signal transduction pathways in human fibroblasts: integral role for extracellular calcium. Arch. Biochem. Biophys. 304: 294-301, 1993. PubMed: 7686736

McAllister BS, et al. Bradykinin inhibition of EGF- and PDGF-induced DNA synthesis in human fibroblasts. Am. J. Physiol. 265: C477-C484, 1993. PubMed: 8396328