CAR (ATCC® CCL-71)

Organism: Carassius auratus, goldfish  / 

Permits and Restrictions

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Organism Carassius auratus, goldfish
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Storage Conditions liquid nitrogen vapor temperature
Virus Resistance
poliovirus 1; herpes simplex virus
Complete Growth Medium The base medium for this cell line is Minimum essential medium (Eagle) in Hanks' BSS containing 1.0 mM sodium pyruvate and 0.1 mM nonessential amino acids . To make the complete growth medium add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 0.35 g/L sodium bicarbonate
  • Subculturing
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
    Medium Renewal: Twice per week
    Cryopreservation
    Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor temperature
    Culture Conditions
    Temperature: 25.0°C
    Name of Depositor L Moewus-Kobb
    References

    . . Ann. N.Y. Acad. Sci. 126: 344, 1964.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    . . Ann. N.Y. Acad. Sci. 126: 344, 1964.