K:Molv NIH/3T3 (ATCC® CRL-6361)

Organism: Mus musculus, mouse  /  Disease: sarcoma

Permits and Restrictions

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Organism Mus musculus, mouse
Product Format frozen
Culture Properties adherent
Biosafety Level 2  [Cells are infected with the Kirsten strain of Moloney murine sarcoma virus]
Disease sarcoma
Age embryo
Strain NIH/Swiss
Storage Conditions liquid nitrogen vapor phase
Comments
The cells are infected with the Kirsten strain of Moloney murine sarcoma virus.
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:25 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

 [Cells are infected with the Kirsten strain of Moloney murine sarcoma virus]