3T6-Swiss albino (ATCC® CCL-96)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age embryo
Applications
transfection host
Karyotype Karyotype unstable within stemline range (78-81). Approximately 21% of the cells contained an extra large chromosome with a terminal centromere and 21% contained minute chromosomes.
Derivation
The 3T6 cell line is a collagen and hyaluronic acid secreting line established by G. Todaro and H. Green in 1963 from disaggregated Swiss mouse embryos.
Genes Expressed
collagen; hyaluronic acid
Cellular Products
collagen; hyaluronic acid
Virus Resistance
poliovirus 2
Comments
The 3T6 cell line is a collagen and hyaluronic acid secreting line established by G. Todaro and H. Green in 1963 from disaggregated Swiss mouse embryos.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor H Green
Year of Origin 1963
References

Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244

Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482

Green H, et al. Differentiated cell types and the regulation of collagen synthesis. Nature 212: 631-633, 1966. PubMed: 5971697

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244

Vogt M, Dulbecco R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology 16: 41-51, 1962. PubMed: 13926482

Green H, et al. Differentiated cell types and the regulation of collagen synthesis. Nature 212: 631-633, 1966. PubMed: 5971697