M-MSV-BALB/3T3 (ATCC® CCL-163.2)

Organism: Mus musculus, mouse  /  Cell Type: fibroblastMoloney murine sarcoma virus (Mo-MSV) transformed  / 

Organism Mus musculus, mouse
Cell Type fibroblastMoloney murine sarcoma virus (Mo-MSV) transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 14 to 17 day gestation embryo
Strain BALB/c
Applications
Do not produce M-MSV.
Derived from BALB/3T3 (CCL-163).
Derivation
Derived from BALB/3T3 (CCL-163).
Antigen Expression
H-2d
Genes Expressed
H-2d
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Do not produce M-MSV.
Derived from BALB/3T3 (CCL-163).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10%
Subculturing
Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let the culture stand at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37.0°C
Name of Depositor S Aaronson
References

Aaronson SA, Rowe SP. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology 42: 9-19, 1970. PubMed: 4318987

Basic Documentation
References

Aaronson SA, Rowe SP. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology 42: 9-19, 1970. PubMed: 4318987