ICR-2A (RPH68.2A) [Haploid Frog] (ATCC® CCL-145)

Organism: Rana pipiens, frog, grass  / 

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Organism Rana pipiens, frog, grass
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age embryo
Storage Conditions liquid nitrogen vapor phase
Karyotype haploid; number 10 chromosome has a secondary constriction
Virus Susceptibility Frog virus 4 , Frog virus 4
Vesicular stomatitis virus
Human poliovirus 1
Herpes simplex virus
Vaccinia virus
Comments
For diploid cells, see ICR 134 (ATCC CCL-128).
Complete Growth Medium Ham's F-12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 40% double distilled water and 10% fetal bovine serum.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 25C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Incubate at 25.0C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 25.0°C
Name of Depositor J Freed, L Mezger-Freed
References

. . Methods Cell Physiol. 4: 20-46, 1970.

Freed JJ, Mezger-Freed L. Stable haploid cultured cell lines from frog embryos. Proc. Natl. Acad. Sci. USA 65: 337-344, 1970. PubMed: 5263768

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

. . Methods Cell Physiol. 4: 20-46, 1970.

Freed JJ, Mezger-Freed L. Stable haploid cultured cell lines from frog embryos. Proc. Natl. Acad. Sci. USA 65: 337-344, 1970. PubMed: 5263768