NCI-H295 [H295] (ATCC® CRL-10296)

Organism: Homo sapiens, human  /  Disease: adrenocortical carcinoma

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Organism Homo sapiens, human
Product Format frozen
Morphology epithelial
Culture Properties suspension, with some loosely adherent cells, suspension with some loosely attached cells
Biosafety Level 1
Disease adrenocortical carcinoma
Age 48 years
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 62; range = 55 to 64; hypertriploid. Twenty-three marker chromosomes were common to most cells: der(2)t(2;18)(p25;p11), del(3)(q21), der(10)t(10;13)(p15;q11), der(7)t(7;7)(q36;p13), del(7)(q11),; der(8)t(7;8)(p13;p23), del(8)(p12), del(9)(p22), del(9)(p13), del(11)(p12), del(12)(p12), t(13q18q) and 11 others. The del(9)(p13) was paired; normal N13 was absent. N15 had three copies and N22 had four copies.; A total of 65 marker chromosomes were detected in 38 metaphases examined.; A minor subline that occurred in about 16% of the cells was characterized by the presence of del(M5)(7p11) replacing M5, by the single N17 (instead of paired N17) and by the presence of an additional M42.
Clinical Data
female
Black
48 years
Genes Expressed
steroid hormones
Complete Growth Medium HITES medium supplemented with 2% fetal bovine serum
    HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows:
  • Dulbecco's medium : Ham's F12, 50:50 mix (ATCC 30-2006)
  • Insulin 0.005 mg/ml
  • Transferrin 0.01 mg/ml
  • Sodium selenite 30 nM
  • Hydrocortisone 10 nM
  • beta-estradiol 10 nM
  • HEPES 10 mM
  • L-glutamine 2 mM (in addition to that in the base medium)
  • fetal bovine serum 2%
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cells with some loosely attached cells. Shake the flask to dislodge the cells and transfer to a new flask.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 8% fetal bovine serum and 5% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Name of Depositor AF Gazdar
References

Gazdar AF, et al. Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res. 50: 5488-5496, 1990. PubMed: 2386954

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gazdar AF, et al. Establishment and characterization of a human adrenocortical carcinoma cell line that expresses multiple pathways of steroid biosynthesis. Cancer Res. 50: 5488-5496, 1990. PubMed: 2386954