MOVAS (ATCC® CRL-2797)

Organism: Mus musculus, mouse  /  Cell Type: immortalized with SV40 large T antigen  /  Tissue: aorta/smooth muscle  / 

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Organism Mus musculus, mouse
Tissue
aorta/smooth muscle
Cell Type immortalized with SV40 large T antigen
Product Format frozen
Morphology spindle-shaped
Culture Properties adherent
Biosafety Level 2
Age adult
Strain C57BL/6
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
Primary vascular aortic smooth muscle cells isolated by collagenase - elastase digestions in December 1999 were transduced with retrovirus encoding the SV40 large T antigen as well as the gene for neomycin resistance. Clones were selected in the presence of 0.4 mg/ml G418 and subcloned by limiting dilution.
Genes Expressed
actin; smooth muscle alpha actin,calponin 1; basic; smooth muscle,desmin,smooth muscle protein 22 (SM22 alpha)
Cellular Products
actin; smooth muscle alpha actin
calponin 1; basic; smooth muscle
desmin
smooth muscle protein 22 (SM22 alpha)
Comments
Primary vascular aortic smooth muscle cells isolated by collagenase - elastase digestions in December 1999 were transduced with retrovirus encoding the SV40 large T antigen as well as the gene for neomycin resistance. Clones were selected in the presence of 0.4 mg/ml G418 and subcloned by limiting dilution.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • 0.2 mg/ml G -418
  • fetal bovine serum to a final concentration of 10%
  • Subculturing

    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 4 X 10(3) to 2 X 10(4) viable cells/sq. cm is recommended.
    6. Incubate cultures at 37°C.
    Interval: Maintain cultures at a cell concentration between 1 X 104 and 1 X 105 cells/cm2
    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
    Medium Renewal: Two to three times weekly
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Population Doubling Time 15 hours
    Name of Depositor M Husain
    Year of Origin December, 1999
    References

    Afroze T, Husain M. c-Myb-binding sites mediate G(1)/S-associated repression of the plasma membrane Ca(2+)-ATPase-1 promoter. J. Biol. Chem. 275: 9062-9069, 2000. PubMed: 10722757

    Afroze T, et al. Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in the vascular smooth muscle cell cycle. Am. J. Physiol. Cell Physiol. : C88-C95, 2003. PubMed: 12660151

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Other Documentation
    References

    Afroze T, Husain M. c-Myb-binding sites mediate G(1)/S-associated repression of the plasma membrane Ca(2+)-ATPase-1 promoter. J. Biol. Chem. 275: 9062-9069, 2000. PubMed: 10722757

    Afroze T, et al. Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in the vascular smooth muscle cell cycle. Am. J. Physiol. Cell Physiol. : C88-C95, 2003. PubMed: 12660151