SV40LT-SMC Clone HEP-SA (ATCC® CRL-2018)

Organism: Rattus norvegicus, rat  /  Cell Type: SV40 transfected  / 

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Organism Rattus norvegicus, rat
Cell Type SV40 transfected
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age 3 months
Strain Sprague-Dawley
Applications
This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.
Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Storage Conditions liqid nitrogen vapor temperature
Derivation
This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.
The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Genes Expressed
alpha actin; large T antigen
Cellular Products
alpha actin; large T antigen
Comments
This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.
The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin.
Large T antigen is detectable in the nuclei.
Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 10% Bovine Calf Serum and 200 mcg/mL G418
Subculturing
Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove. Allow the cells to sit at 37C (or at room temperature) until the cells detach (usually 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Name of Depositor CF Reilly, R McFall
References

Reilly CF. Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin. J. Cell. Physiol. 142: 342-351, 1990. PubMed: 2154504

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reilly CF. Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin. J. Cell. Physiol. 142: 342-351, 1990. PubMed: 2154504