BC3H1 (ATCC® CRL-1443)

Organism: Mus musculus, mouse  /  Cell Type: methylnitrosourea induced  / 

Organism Mus musculus, mouse
Cell Type methylnitrosourea induced
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Strain C3H
Applications
Recent data suggest that BC3H1 cells may more closely resemble cells in an arrested state of skeletal muscle differentiation than smooth muscle cells.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression
H-2k
Receptor Expression
acetylcholine
Genes Expressed
adenylate phosphokinase; creatine phosphokinase,H-2k
Cellular Products
adenylate phosphokinase; creatine phosphokinase
Comments
Recent data suggest that BC3H1 cells may more closely resemble cells in an arrested state of skeletal muscle differentiation than smooth muscle cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor W Carlisle
References

Schubert D, et al. Characterization of a unique muscle cell line. J. Cell Biol. 61: 398-413, 1974. PubMed: 4363958

Taubman MB, et al. The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. J. Cell Biol. 108: 1799-1806, 1989. PubMed: 2715180

Patrick J, et al. Acetylcholine receptor metabolism in a nonfusing muscle cell line. J. Biol. Chem. 252: 2143-2153, 1977. PubMed: 845167

Basic Documentation
References

Schubert D, et al. Characterization of a unique muscle cell line. J. Cell Biol. 61: 398-413, 1974. PubMed: 4363958

Taubman MB, et al. The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation. J. Cell Biol. 108: 1799-1806, 1989. PubMed: 2715180

Patrick J, et al. Acetylcholine receptor metabolism in a nonfusing muscle cell line. J. Biol. Chem. 252: 2143-2153, 1977. PubMed: 845167