NOR-10 (ATCC® CCL-197)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

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Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 30 days
Strain C57BL/10
Applications
This may be used as a matched control for ATCC CCL-198 (BLO-1).
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression
H-2b
Genes Expressed
H-2b
Comments
This may be used as a matched control for ATCC CCL-198 (BLO-1).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.1% trypsin for about 10 minutes, dislodge cells and centrifuge to remove from trypsin. Add fresh medium to cell pellet, resuspend cells in fresh medium and dispense into new flasks. Subculture every 3 to 5 days.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor BC Starcher
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation