D10.G4.1 (ATCC® TIB-224)

Organism: Mus musculus, mouse  /  Cell Type: helper T lymphocyte (Th-2)  /  Tissue: lymph node  / 

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Organism Mus musculus, mouse
Tissue lymph node
Cell Type helper T lymphocyte (Th-2)
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age adult
Gender male
Strain AKR/J
Applications
D10.G4.1 has been used extensively to study activation of T cells. These cells can also be used as a reliable and sensitive assay for IL-1 (interleukin-1) response, since D10 cells will proliferate only minimally to con A in the absence of IL-1 or feeder cells.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Antigen Expression
H-2k
Receptor Expression

interleukin 1 (IL-1), expressed; interleukin 1 (IL-1)

Genes Expressed
interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5)
Cellular Products
interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5)
Comments
The cells are specific for egg white conalbumin (Sigma No. C0755) and alloreactive to I-A b, v.
Supernatant media from Con A stimulated D10.G4.1 cells contains IL-4 and IL-5.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 10% T-STIM with Con A (rat IL-2 culture supplement ) Becton Dickinson, Catalog No. 354115)
  • 10% fetal bovine serum
  • 0.05 mM 2-mercaptoethanol
  • 10 pg/ml mouse IL-1 alpha (R&D Systems, Catalog No. 400ML)

Subculturing
Subculture actively growing suspension cultures before they have reached 1 X 106 cells/mL or the IL-1 and IL-2 will be rapidly depleted and the cells will lose viability. Use inoculation densities of 2 X 105 viable cells/mL.
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 5% (v/v) DMSO.
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor C Janeway, J Kaye, P Conrad
References

Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804

Kaye J, Janeway CA J. Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 3: 175-182, 1984. PubMed: 6436584

Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524

Kaye J, et al. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836-856, 1983. PubMed: 6193236

Kaye J, Janeway CA Jr.. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T cell clone blocks activation by either allogeneic Ia or antigen and self-Ia. J. Exp. Med. 159: 1397-1412, 1984. PubMed: 6232337

Kaye J, et al. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J. Immunol. 133: 1339-1345, 1984. PubMed: 6235287

Kaye J, Janeway CA Jr.. The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J. Immunol. 133: 2291-2300, 1984. PubMed: 6237148

Swain SL, et al. Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J. Immunol. 140: 4224-4230, 1988. PubMed: 2453572

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804

Kaye J, Janeway CA J. Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 3: 175-182, 1984. PubMed: 6436584

Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524

Kaye J, et al. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836-856, 1983. PubMed: 6193236

Kaye J, Janeway CA Jr.. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T cell clone blocks activation by either allogeneic Ia or antigen and self-Ia. J. Exp. Med. 159: 1397-1412, 1984. PubMed: 6232337

Kaye J, et al. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J. Immunol. 133: 1339-1345, 1984. PubMed: 6235287

Kaye J, Janeway CA Jr.. The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J. Immunol. 133: 2291-2300, 1984. PubMed: 6237148

Swain SL, et al. Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J. Immunol. 140: 4224-4230, 1988. PubMed: 2453572